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Replication kinetics of GRL-286-resistant HIV-1. MT-4 cells (2.4 × 105) were exposed to an HIV-1286-1 μM or a wild-type HIV-1NL4-3 preparation containing 30 ng p24 in six-well culture plates for 3 h, and the MT-4 cells were divided into three fractions, each of which was cultured with or without GRL-286 (104 MT-4 cells/ml and drug concentrations of 0, 0.01, and 0.1 μM). The amounts of p24 were measured every 2 days for up to 9 days.
