TABLE 2.
Primer | Sequence (5′-3′)a |
---|---|
bpeR-RT-F | GCGAAATCCTGATCTGGTG |
bpeR-RT-R | TGAACAGGATGCTGAACACG |
bpeB-F1-RT | GGCCTCGACAACTTCCTGTA |
bpeB-R1-RT | TTGTTCTGCACCTGAACCTG |
Bp23S-F | GTAGACCCGAAACCAGGTGA |
Bp23S-R | CACCCCTATCCACAGCTCAT |
bpeR-UP-For | CGTCGTACTGCTGCTTGCTG |
bpeR-UP-Rev-GM | tcagagcgcttttgaagctaattcgCTTCCTCCTTCGTGCGTCTG |
bpeR-DN-For-GM | aggaacttcaagatccccaattcgGATGCGCAAGGACGACTGAG |
bpeR-DN-Rev | ACACGAGACGCACGCTGAAC |
GmFRT-UP | cgaattagcttcaaaagcgctctga |
GmFRT-DN | cgaattggggatcttgaagtacct |
bpeR-check-For | CAGACGCACGAAGGAGGAAG |
bpeR-check-Rev | CTCAGTCGTCCTTGCGCATC |
bpeA-For | GTACGAGCGCCTATCTGGTC |
bpeA-Rev-FRT | aggaacttcaagatccccaattcgGCTCTCGACCACCCAGTTCT |
oprB-For-FRT | tcagagcgcttttgaagctaattcgAGATCGCGCTGAAGAACAAC |
oprB-Rev | CTCTGGATCGCCTTCTCGTA |
bpeR-For primer | GGAGGGAAATCAGCAGAGAC |
bpeR-Rev primer | CGTCACGTGAGTTTCGCGAC |
bpeR-SOEing-For | ATGGCCAGACGCACGAAGGAG |
bpeR-SOEing-Rev | CGTCACGTGAGTTTCGCGAC |
P1-SOEing-For-EcoRV | GGGATATCGAATTCACGAACCCAGTTGAC |
P1-bpeR-SOEing-Rev | CTCCTTCGTGCGTCTGGCCATGTCGAATCCTTCTTGTGAATC |
Uppercase letters denote chromosome- or plasmid-specific sequences, and lowercase letters denote FRT cassette-specific sequences.