TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| bpeR-RT-F | GCGAAATCCTGATCTGGTG |
| bpeR-RT-R | TGAACAGGATGCTGAACACG |
| bpeB-F1-RT | GGCCTCGACAACTTCCTGTA |
| bpeB-R1-RT | TTGTTCTGCACCTGAACCTG |
| Bp23S-F | GTAGACCCGAAACCAGGTGA |
| Bp23S-R | CACCCCTATCCACAGCTCAT |
| bpeR-UP-For | CGTCGTACTGCTGCTTGCTG |
| bpeR-UP-Rev-GM | tcagagcgcttttgaagctaattcgCTTCCTCCTTCGTGCGTCTG |
| bpeR-DN-For-GM | aggaacttcaagatccccaattcgGATGCGCAAGGACGACTGAG |
| bpeR-DN-Rev | ACACGAGACGCACGCTGAAC |
| GmFRT-UP | cgaattagcttcaaaagcgctctga |
| GmFRT-DN | cgaattggggatcttgaagtacct |
| bpeR-check-For | CAGACGCACGAAGGAGGAAG |
| bpeR-check-Rev | CTCAGTCGTCCTTGCGCATC |
| bpeA-For | GTACGAGCGCCTATCTGGTC |
| bpeA-Rev-FRT | aggaacttcaagatccccaattcgGCTCTCGACCACCCAGTTCT |
| oprB-For-FRT | tcagagcgcttttgaagctaattcgAGATCGCGCTGAAGAACAAC |
| oprB-Rev | CTCTGGATCGCCTTCTCGTA |
| bpeR-For primer | GGAGGGAAATCAGCAGAGAC |
| bpeR-Rev primer | CGTCACGTGAGTTTCGCGAC |
| bpeR-SOEing-For | ATGGCCAGACGCACGAAGGAG |
| bpeR-SOEing-Rev | CGTCACGTGAGTTTCGCGAC |
| P1-SOEing-For-EcoRV | GGGATATCGAATTCACGAACCCAGTTGAC |
| P1-bpeR-SOEing-Rev | CTCCTTCGTGCGTCTGGCCATGTCGAATCCTTCTTGTGAATC |
a
Uppercase letters denote chromosome- or plasmid-specific sequences, and lowercase letters denote FRT cassette-specific sequences.