(A) A schematic representation of the air-liquid interface (x, alveolar compartment; y, endovascular compartment); (B) the decline in the transgression of dextran blue into the endovascular compartment as a function of time, reflecting the attainment of cellular confluence. (C to F) Confocal images at 12, 16, 20, and 24 h postinoculation are shown; the lower panes depict the corresponding cross-sectional images. Scale bar, 10 μm. (G) The kinetics of galactomannan in infected but untreated inserts. The solid line represents the time course of galactomannan in the endovascular compartment, whereas the dashed line is the time course in the alveolar compartment. Data are means ± standard deviations of three inserts.