TABLE 2.

Sda reduces the efficiency of sporulation when cells are grown in LB medium lacking manganese^a

Relevant genotype	Strain	Mn(II) concn (μM)	Total CFU ml−1	No. of spores ml−1	Sporulation frequencyb
Wild type	BB825	0	1.2 × 109	5.9 × 104	4.9 × 10−5
Δsda	BB827	0	6.5 × 108	8.4 × 105	1.3 × 10−3
Wild type	BB825	100	9.7 × 108	5.3 × 108	0.55
Δsda	BB827	100	8.5 × 108	4.6 × 108	0.54

^aData are from the same experiment shown in Fig. 1. Similar sporulation data were obtained in 10 additional independent experiments (data not shown).

^bThe sporulation frequency is the ratio of spores ml⁻¹ to total CFU ml⁻¹.