FIG. 3.
Evaluation of gene replacement (ΔehbF::pac and ΔehbO::pac) and deletion (ΔehbF) mutants of the conserved integral membrane proteins in rich (McCA, closed symbols) and minimal (McA, open symbols) media. (A) Growth of the ΔehbF::pac strain S40 (triangles) and the parental strain S2 (circles). (B) Growth of the ΔehbO::pac strain S42 (squares) and the parental strain S2 (circles). For panels A and B, the experiment was begun with ∼2 × 106 cells upon inoculation into prewarmed media. (C) Growth of the ΔehbF strain S940 (triangles), strain S943 containing the ΔehbF mutation complemented with pMEV2+ehbF (squares), and the parental strain S900 (circles). In these experiments, prewarmed McA media were inoculated with ∼105 cells. Growth curves are representative of experiments that were performed at least twice in duplicate.