TABLE 1.

Quantitive analysis of irregular chromosome appearance from mid-log-phase culture^a

Temp and strain genotype	Total no. of cells counted	% of cells:
		Anucleate	With “CUT” phenotype	With uneven chromosome distribution	With increased/decreased DNA content	Big/smallb
30°C
WT	911	0.3	NOc	NO	0.2/NO	NO
Δsmc	1,258	9.4	NO	0.3	0.3/NO	NO
smc::Tn917	881	10.4	NO	0.3	0.3/NO	NO
smc::Tn917 pCXΩsmc	1,316	2.3	NO	0.5	0.3/NO	0.1/NO
ΔspoIIIE	975	0.7	NO	0.4	0.5/NO	NO/0.1
smc::Tn917 ΔspoIIIE	802	4.8	1.9	10.7	3.2/6.1	1.6/4.5
37°C
WT	1,023	0.3	NO	0.7	0.6/NO	NO
Δsmc	1,144	7.9	NO	0.7	0.5/NO	NO/0.3
smc::Tn917	851	7.2	NO	0.8	0.7/0.2	NO/0.2
smc::Tn917 pCXΩsmc	1,216	1.8	NO	0.7	0.7/0.3	NO/0.3
ΔspoIIIE	732	0.4	NO	0.7	0.8/NO	NO/0.1
smc::Tn917 ΔspoIIIE	826	6.4	1.7	20.8	5.3/6.2	2.9/4.5
42°C
WT	987	0.3	NO	1.5	1.5/0.8	NO/0.7
Δsmc	1,240	2.5	NO	1.4	1.8/1.0	NO/0.9
smc::Tn917	922	3.5	NO	1.5	2.0/0.9	NO/1.1
smc::Tn917 pCXΩsmc	1,232	1.7	NO	1.5	1.9/1.2	0.3/1.1
ΔspoIIIE	890	0.3	NO	1.6	2.1/1.0	NO/0.9
smc::Tn917 ΔspoIIIE	681	6.2	2.0	28.5	5.3/11.2	4.0/7.0

^aData were summarized from three independent experiments.

^bBig cells are ≥1.5 μm in diameter, and small cells are ≤0.5 μm in diameter.

^cNO, not observed within the total number of cells counted.