FIG. 3.

Effect of the growth phase on the expression of the cutBCA operons in the WT and the ΔCutRmt strains of Mycobacterium sp. strain JC1. The WT (black bar) and ΔCutRmt (gray bar) strains were grown to an OD₆₀₀ of 0.3 (early log), 0.6 (mid log), and 2.3 (early stationary) in SMB medium supplemented with 0.2% (wt/vol) glucose. For the late stationary culture, cells which had reached the early stationary phase, were further grown for additional 50 h. (A) CO-DH activities; (B) promoter activities of cutB1; (C) promoter activities of cutB2. All values provided are the means of two independent determinations. Error bars represent the standard deviationd from the mean.