FIG. 4.

Expression of the cutB1 gene in Mycobacterium sp. strain JC1 under glucose-limiting conditions. The WT strain carrying pCUTB1LACZ2 was grown in SMB medium supplemented with 0.1% (wt/vol) glucose to an OD₆₀₀ of 0.5. The culture was divided into two groups. The control group (black bar) was grown for additional 5 h under the same conditions. In the case of the experimental group (gray bar), methyl-α-d-glucopyranoside (MGP), a nonmetabolizable glucose homolog, was added to the culture to a final concentration of 10% (wt/vol), and the culture was further grown for additional 5 h. The β-galactosidase activities in cells grown to an OD₆₀₀ of 0.5 (0 h) and for 5 h thereafter (5 h) were measured to determine expression of cutB1. Error bars indicate the standard deviations of the results obtained from two independent determinations.