FIG. 6.

Promoter activities from the serially deleted and point-mutated promoters of cutB2 in Mycobacterium sp. strain JC1 grown on glucose. The strains harboring the corresponding transcriptional fusion plasmids were grown on SMB medium with 0.2% (wt/vol) glucose either to mid-exponential phase (black bar) or to late stationary phase (gray bar) as described in Fig. 3. pCUTB2up461, pCUTB2up82, and pCUTB2up58 contain the 461-, 82-, and 58-bp upstream sequences of cutB2, respectively. pCUTB2CBSPM has the same construct as pCUTB2up461 except that the CRP-binding sequence is point mutated. The β-galactosidase activities in cells were measured to determine expression of cutB2. Error bars indicate the standard deviations of the results obtained from two independent determinations.