TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| Mycobacterium sp. strain JC1 | Wild type (DSM 3803) | 5, 37 |
| Mycobacterium sp. strain ΔCutR | cutR deletion mutant derived from Mycobacterium sp. strain JC1 | This study |
| E. coli DH5α | supE44 lac16 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 Δthi relA1 | 14 |
| Plasmids | ||
| pKO | Hygr; sacB, suicide vector | 7 |
| pNBV1 | Hygr; 5.8-kb plasmid derived from p16R1 | 12 |
| pCV77 | Kmr; promoterless lacZ | MediImmune |
| pNC | Hygr; promoterless lacZ | 38 |
| pTS19 | pBluescript II SK(+) containing the 995-bp cutR and cutB1 of Mycobacterium sp. strain JC1 | 38 |
| pTS49 | pBluescript II SK(+) containing the 873-bp cutB2 of Mycobacterium sp. strain JC1 | 38 |
| pSJ1 | pBluescript II KS(+)::1,304-bp EcoRI-BamHI fragment containing cutR | This study |
| pSJ2 | pBluescript II KS(+)::1,039-bp EcoRI-BamHI fragment containing the ΔcutR mutation | This study |
| pSJ3 | pKO::1,051-bp HindIII-BamHI fragment containing the ΔcutR mutation | This study |
| pCUTR | pNBV1::1,316-bp HindIII-BamHI fragment containing cutR | This study |
| pCUTB1LACZ2 | pNC::1,192-bp ClaI-XbaI fragment containing the cutB1 and cutR intergenic region | 38 |
| pCUTB2LACZ2 | pNC::872-bp ClaI-XbaI fragment containing the cutB2 and orf1 intergenic region | 38 |
| pCUTB2up461 | pNC::461-bp upstream region of cutB2 | This study |
| pCUTB2up82 | pNC::82-bp upstream region of cutB2 | This study |
| pCUTB2up58 | pNC::58-bp upstream region of cutB2 | This study |
| pCUTB2CBSPM | The same construct as pCUTB2up461 except point mutations in the putative CRP-binding site upstream of cutB2 | This study |
| pBScutB2 | pBluescript II KS(+)::899-bp BamHI-ClaI fragment from pCUTB2LACZ2 | This study |
a
Hygr, hygromycin resistance; Kmr, kanamycin resistance.