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. 2010 May 28;192(15):3951–3960. doi: 10.1128/JB.00098-10

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FIG. 4. — Determination of the specificity of Vsr endonucleases expressed by N. gonorrhoeae FA1090. (A) Specificity of V.NgoAXIII. (B) Specificity of V.NgoAXIV. Lane 1, control substrate DNA without T:G mismatch; lane M, GeneRuler 50-bp DNA ladder; lane 2, substrate DNA M.NgoAI-sub containing the sequence AGTGCT/AGCGCT; lane 3, substrate DNA M.NgoAII-sub containing the sequence GGTC/GGCC; lane 4, substrate DNA M.NgoAIII-first-sub containing the sequence TCGCGG/CCGCGG; lane 5, substrate DNA M.NgoAIII-second-sub containing the sequence CTGCGG/CCGCGG; lane 6, substrate DNA M.NgoAIII-third-sub containing the sequence CCGTGG/CCGCGG; lane 7, substrate DNA M.NgoAIV-sub containing the sequence GTCGGC/GCCGGC; lane 8, substrate DNA M.NgoAVII-sub containing the sequence GTGGC/GCCGC; lane 9, substrate DNA M.NgoAORF675P-sub containing the sequence GGCTTC/GGAGCC; lane 10, substrate DNA M.NgoAORF302P-sub containing the sequence ATCGGC/GCCGGT; lane 11, substrate DNA M.NgoAORF1175P-sub containing the sequence CTGG/CCGG. The T:G mismatch resulting from deamination of m5C to thymine is in boldface. Arrows indicate substrate (S) and reaction products (P1 and P2) obtained after cleavage by purified Vsr endonucleases.