Cell cycle parameters for wild-type (Wt) (E. coli MG1655), ΩSm, oriCIVc, and oriCIVc* strains. Wt, ΩSm, oriCIVc, and oriCIVc* cells were grown at 37°C in minimal medium supplemented with glycerol, glucose, or glucose plus Casamino Acids (CAA). Cells were treated with rifampin and cephalexin (Materials and Methods) prior to flow cytometric analysis.