TABLE 3.
Relative P1 transduction of argE::Tn10 and dam13::Tn9 into strains with mutated oriC regionsa
| Recipient | Mnemonic | Minichromosome phenotypeb | Cmr/Tcr ratioc |
|---|---|---|---|
| WM2482 | Wild-type | ++ | 0.9 |
| WM2759 (oriC160) | Deletion of 77 bp to the right of R4 | + | 1.0 |
| WM2762 (oriC13) | Scrambled R2 | + | 0.9 |
| WM2764 (oriC15) | Scrambled R4 | − | 9.0 × 10−4 |
| WM2844 (oriC17) | Scrambled R5 | − | 3.3 × 10−4 |
| WM2845 (oriC162) | Addition of 14 bp between R3 and R4 | − | 2.6 × 10−4 |
| ALO3270 (oriC-I3,I6) | G3T in I2 and G3→T in I3 | +++ | 1.4 |
| ALO3699 (ΩSm) | ΩSm inserted next to oriC | ND | 1.2 |
| ALO3150 (oriCIVc*) | oriC replaced by oriCIVc* | ND | 2.2 × 10−4 |
| ALO3702 (oriCIVc) | oriC replaced by oriCIVc | ||
| Incubated at 37°Cd | ND | 1.0 × 10−3 | |
| Incubated at 42°Cd | ND | 1.3 × 10−4 |
Plates were inspected after overnight incubation at 37°C. Strains with oriC mutations oriC14, oriC21, oriC131, oriC132, and oriC136 (84) behaved similar to wild-type (wt), oriC160, and oriC13 strains.
Data from reference 84, except for the oriC-I3,I6 mutant (data from reference 23). Symbols: +++, increased oriC function; ++, normal oriC function; +, reduced oriC function; −, inactive oriC. ND, not determined.
Transduction efficiency of argE::Tn9 into the recipient was 1 × 10−6 to 2 × 10−6 in all cases except for oriC15 and oriC162 strains, where it was approximately 5-fold lower.
There were about as many microcolonies as seen on the plates containing tetracycline. These microcolonies could not really restreak and did not appear when the plates were incubated at 42°C.