TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U139 rpsL150 flbB5301 ptsF25 deoC1 thi-1 rbsR relA | 6 |
| JP313 | MC4100 Δara-174 | Laboratory collection |
| LMD439 | JP313 att λ::hasDE | 11 |
| MC4100 ΔsecB | 35 | |
| DH5α | ||
| Plasmids | ||
| pAM238 | Low-copy-number expression vector; Spcr | Laboratory collection |
| pAM-HasA | Wild-type HasA in pAM238 | 30 |
| pAM-HasAE148A, D167A | 45 | |
| pAM-HasAX | pAM-HasA mutant derivatives with X representing the positions of pentapeptide insertions | 30 |
| pAM-HasA(1-174) | HasA shorn of its 14 C-terminal amino acids in pAM238 | 7 |
| pAM-HasA(133-188) | 56 C-terminal amino acids of HasA in pAM238 | 7 |
| pTrc99A | Expression vector; Apr | Pharmacia |
| pTrc99A-HasA | 11 | |
| pTrc99A-HasA(1-174) | 7 | |
| pTrc99A-HasA(133-188) | 7 | |
| pTrc99A-HasA(1-174)X | This work | |
| pTrc99A-HasA(1-174)E148A, D167A | This work | |
| pTrc99C | Expression vector; Cmr | 11 |
| pTrc99C-HasA | 11 | |
| pTrc99C-HasA(1-174) | 7 | |
| pTrc99C-HasA(133-188) | This work | |
| pTrc99C-HasA(1-174)X | This work | |
| pTrc99C-HasA(1-174)E148A, D167A | This work | |
| pSyc150 | hasDE in pACYC184; Cmr | 26 |
| pAM-HasISRADE | hasISRADE in pAM238 | 17 |
| pBAD24-HisHasDE | 6His-HasD and HasE in pBAD24 | P. Delepelaire, personal communication |