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. 2010 May 28;192(15):4037–4044. doi: 10.1128/JB.00386-10

FIG. 4.

FIG. 4.

Construction of the Rv0241c deletion mutant. (A) Chromosomal organization of the region. The deletion zone is drawn. Arrows indicate the primers used for PCR screening. (B) PCR confirmation of deletion strains. The primers indicated in panel A were used to amplify the Rv0241c region. Wild-type strains gave a product of 2.7 kb, the deletion strains gave a product of 2.2 kb. Lane 1, wt strain; lanes 2 to 5, deletion strains; lane 6, negative control; lane M, 1-kb markers. Deletions were verified by Southern hybridization.