FIG. 5.

Analysis of the mycolic acid content of M. tuberculosis ΔRv0241c. (A) TLC of the extractable lipids on silica gel G-60 plate using CHCl₃/CH₃OH/H₂O (60/16/2) as the eluent. Visualization by spraying with anthrone-H₂SO₄ and charring. No differences between the two strains were visible using another eluent (CHCl₃/CH₃OH, 9/1). PAT, polyacyltrehalose; TDM, trehalose dimycolates; SL-I, sulfolipid-I; TMM, trehalose monomycolates; PIMs, phosphatidylinositol mannosides. Standards of TDM and TMM were deposited. (B) TLC of the mycolic acid methyl esters on a silica gel G-60 plate using petroleum ether-diethyl ether (9/1, five passages) as the eluent. Visualization by spraying with molybdophosphoric acid and charring. α, α-mycolates; M, methoxymycolates; K, ketomycolates. The standards α-, methoxy-, and keto-mycolates were deposited. Identical quantities of total lipids from wt and ΔRv0241c strains were deposited in panels A and B.