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. 2010 Jun 4;192(15):3893–3902. doi: 10.1128/JB.00468-10

FIG. 1.

FIG. 1.

The expression of dnaA is required for CckA and DivL localization to the new cell pole. (A) Distribution of bipolar CckA-CFP and MipZ-YFP (strain AA841) throughout the cell cycle, starting with isolated swarmer cells (between 144 and 438 cells were counted at each time point and for each fluorescent marker; notated as n = 144 to 438 cells, hereafter). A cell cycle schematic is shown (below). (B) Phase contrast and fluorescence images of CckA-CFP localization 90 min after swarmer cells isolation (strain AA847) in the presence of DnaA (with 0.3% xylose) or in its absence (without xylose). (C) Histograms of CckA-CFP and DivL-mCherry localization (strains AA847 and AA920) in the presence of DnaA or in its absence (see description for panel B) (n = 213 to 421 cells). (D) Histograms of monopolar CckA-CFP and DivL-mCherry (strains AA847 and AA920) partitioning between new and old cell poles in the presence of DnaA or in its absence (see description for panel B) (n = 32 to 73 cells). (E) Phase contrast and fluorescence images of DivL-mCherry localization (strain AA920) in the presence of DnaA or in its absence (see description for panel B).