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. 2010 Jun 4;192(15):3893–3902. doi: 10.1128/JB.00468-10

FIG. 2.

FIG. 2.

Proteolytically stable and active CtrA inhibits CckA and DivL localization at the new cell pole. (A) Phase contrast and fluorescence images of CckA-CFP localization 90 min after swarmer cells (strain AA1059) were isolated and allowed to proceed synchronously through the cell cycle in the presence of CtrA(D51E)Δ3Ω or in its absence. A culture of strain AA1059 was incubated for 1 h in the presence of 0.3% xylose [to induce ctrA(D51E)Δ3Ω], and another culture of the same strain was incubated without inducer. Swarmer cells were then isolated from the induced and the uninduced culture, washed, and resuspended in medium containing 0.3% xylose or in the absence of xylose, respectively. (B) Histograms of CckA-CFP and DivL-mCherry (strains AA1059 and AA1046) in the presence of CtrA(D51E)Δ3Ω or in its absence (see description for panel A) (n = 319 to 637 cells). (C) Phase contrast and fluorescence images of DivL-mCherry localization (strain AA1046) in the presence of CtrA(D51E)Δ3Ω or in its absence (see description for panel A). (D) Histograms of monopolar CckA-CFP and DivL-mCherry (strains AA1059 and AA1046) partitioning between new and old cell poles in the presence of CtrA(D51E)Δ3Ω or in its absence (see description for panel A) (n = 12 to 114 cells).