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. 2010 Jun 4;192(15):3893–3902. doi: 10.1128/JB.00468-10

FIG. 3.

FIG. 3.

The DNA replication inhibitor novobiocin inhibits CckA and DivL localization at the new cell pole. (A) Phase contrast and fluorescence images of CckA-CFP and MipZ-YFP subcellular localization (in strain AA841) with or without novobiocin treatment. Synchronized swarmer cells were incubated for 90 min without or with 100 μg/ml novobiocin. (B) Histograms of CckA-CFP and MipZ-YFP (strain AA841) and DivL-mCherry (strain NJH602) localization with or without novobiocin (see description for panel A) (n = 158 to 613 cells). (C) Histograms of monopolar CckA-CFP and DivL-mCherry (strains AA841 and NJH602) partitioning to the new and old cell poles with or without novobiocin (see description for panel A) (n = 5 to 144 cells). (D) Phase contrast and fluorescence images of DivL-mCherry localization (strain NJH602) with or without novobiocin (see description for panel A).