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. 2010 Jun 1;30(15):3842–3852. doi: 10.1128/MCB.01610-09

FIG. 6.

FIG. 6.

Bag1-L overexpression increases cell death of CGNs. (A and B) Primary cerebellar neurons were isolated and cultured in medium containing high K+, which promotes their survival. At day in vitro 6 (DIV6), CGNs were transfected with Bag1-L, Bag1-LΔBD, or empty vector together with GFP to mark transfected cells. Cell death was measured after 48 h (including ±12 h of K+ withdrawal, which induces apoptosis) and is shown as a percentage of total cells analyzed. Representative images of each condition indicating normal nuclei (white arrows) and apoptotic nuclei (blue arrows) are shown. An asterisk (P < 0.05) indicates statistical significance; n.s, not statistically significant. (C) A model illustrating the coactivation of apoptotic c-Jun/JNK signaling by Bag1-L in neurons.