TABLE 2.
PCR primers and universal probes for real-time PCR
| Gene product | Probe no. | Primer sequence (5′-3′) |
|
|---|---|---|---|
| Forward | Reverse | ||
| Gusβ | 6 | GATGTGGTCTGTGGCCAAT | TGTGGGTGATCAGCGTCTT |
| α4 | 21 | CAACAGAGCCCGGGTAGA | CGCCCATTGCTCTGTGTAT |
| β2 | 89 | TCCCGAGAGTTGTTACAGCTAA | GCACCAATGTAACCTTGATACCT |
| Rpn13 | 40 | CCCCAGACTGCAGATGAGAT | GCCGCACTGAACATACCC |
| Smp30 | 3 | CGATTCAATGATGGGAAGGT | CGTTTCCTCAGCCATGGTA |
| Cu/ZuSOD | 49 | CCATCAGTATGGGGACAATACA | GGTCTCCAACATGCCTCTCT |
| Gst-mu | 58 | CTACCTTGCCCGAAAGCAC | ATGTCTGCACGGATCCTCTC |
| Gpx2 | 2 | GTTCTCGGCTTCCCTTGC | TTCAGGATCTCCTCGTTCTGA |
| Cyp2a5 | 52 | ACCAAGGACACCAAGTTTCG | AGAGCCCAGCATAGGAAACA |
| Nqo1 | 50 | AGCGTTCGGTATTACGATCC | AGTACAATCAGGGCTCTTCTCG |
| Ercc3 | 6 | CGGGTACTCAGAGCCAAGAA | CAGGGAGTAGAAAAAGGCATTG |
| Xrcc5 | 29 | GAAGATCACATCAGCATCTCCA | CAGGATTCACACTTCCAACCT |
| Ogg1 | 20 | TTATCATGGCTTCCCAAACC | CCTCAGGTGAGTCTCTGCTTC |
| p19ARF | 106 | GGGTTTTCTTGGTGAAGTTCG | TTGCCCATCATCATCACCT |
| p53 | 60 | GTGAGGCGAGTTGTGGAAAT | TGCCACACAGCAGTGAATG |
| p21Waf1/Cip1 | 21 | TCCACAGCGATATCCAGACA | GGACATCACCAGGATTGGAC |
| p63 | 45 | AGACCTCAGTGACCCCATGT | CTGCTGGTCCATGCTGTTC |
| Noxa | 15 | CAGATGCCTGGGAAGTCG | TGAGCACACTCGTCCTTCAA |
| PUMA | 79 | TTCTCCGGAGTGTTCATGC | TACAGCGGAGGGCATCAG |
| p16INK4a | 42 | CTTCCTGGACACGCTGGT | TCTTGATGTCCCCGCTCTT |
| p15INK4b | 42 | GACACGCTTGTCGTGCTG | TGCTCTTCAGCCAAGTCTACC |