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. 2010 Jun 18;192(16):4122–4133. doi: 10.1128/JB.00132-10

FIG. 5.

FIG. 5.

CDGA differentially downregulates E. chaffeensis surface-exposed proteins. (A) Surface expression of OmpA on a representative bacterium. Host cell-free bacteria (Ec) were stained with rabbit anti-OmpA or anti-NtrX (1:25), followed by Alexa Fluor 555-conjugated anti-rabbit IgG, and with dog anti-E. chaffeensis (Ec), followed by FITC-conjugated anti-dog IgG. The right panels are merged images. Scale bar = 2 μm. (B) Host cell-free E. chaffeensis was incubated with 0.3 mM CDGA (CDGA) or solvent (1% [vol/vol] DMSO; CTL) for 2 h. Proteins were detected in the E. chaffeensis lysates with rabbit antibodies to TRP120, P28, OmpA, VirB6-2, VirB9, and Hsp60.