Activation of cadC-lacZ promoter deletions in V. cholerae. (Left graph) Strains were grown aerobically in LB medium buffered to pH 5.5 with 100 mM MES. (Right graph) Strains were grown anaerobically in LB medium buffered to pH 7.0 with 100 mM Trizma. The strains are as follows, from left to right: KSK2896 (cadC-lacZ), WL737 (−102), WL762 (−91), WL786 (−78), KSK2917 (−63), and KSK2900 (ΔaphB [ΔB]).