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Inorganic acid tolerance assays. (A) Strains were exposed to LB challenge medium at pH 4.0 for 20 min without adaptation. (B) Strains were adapted for 60 min to LB medium at pH 5.7 and then exposed to LB challenge medium at pH 4.0 for 60 min. The strains are as follows, from left to right: C6706 (wt), WL361 (ΔVC0770), WL540 (ΔcadC), WL564 (Δcah), GK954 (ΔaphB), GK1240 (ΔnhaB), and GK1242 (Δclc).
