TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and relevant characteristics (endpoint position) | Reference or source |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| C6706 str2 | El Tor Inaba Smr | Laboratory collection |
| O395 | Classical Ogawa Smr | Laboratory collection |
| GK138 | KSK725ΔaphB | 21 |
| GK142 | KSK262 aphB-lacZ | 21 |
| GK954 | C6706 ΔaphB | This work |
| GK976 | KSK1105 ΔaphB | This work |
| GK1222 | KSK262 Δclc-lacZ | This work |
| GK1224 | GK1222 ΔaphB | This work |
| GK1232 | KSK262 ΔnhaB-lacZ | This work |
| GK1234 | GK1232 ΔaphB | This work |
| GK1240 | C6706 ΔnhaB | This work |
| GK1242 | C6706 Δclc | This work |
| KSK262 | C6706 str2 ΔlacZ3 | 21 |
| KSK725 | C6706 str2 ΔlacZ3 tcpP-lacZ | 21 |
| KSK1105 | C6706 ΔtcpPH promoter | 28 |
| KSK2896 | KSK262 cacC+cadC-lacZ | This work |
| KSK2900 | KSK2896 ΔaphB | This work |
| KSK2917 | KSK262 cacC+cadC-lacZ (−63) | This work |
| WL344 | KSK262 ΔVC0770-lacZ | This work |
| WL346 | WL344 ΔaphB | This work |
| WL361 | C6706 ΔVC0770 | This work |
| WL540 | C6706 ΔcadC | This work |
| WL547 | KSK262 ΔcadC-lacZ | This work |
| WL558 | KSK262 Δcah-lacZ | This work |
| WL562 | WL558 ΔaphB | This work |
| WL564 | C6706 Δcah | This work |
| WL737 | KSK262 cacC+cadC-lacZ (−102) | This work |
| WL762 | KSK262 cacC+cadC-lacZ (−91) | This work |
| WL786 | KSK262 cacC+cadC-lacZ (−78) | This work |
| WL972 | WL547 Δfnr ΔarcA | This work |
| E. coli | ||
| KSK2925 | MC1061 cadC-lacZ pMF3 | This work |
| KSK2926 | KSK2925 pGKK263 | This work |
| Plasmids | ||
| pGKK263 | aphB in pMF3 | This work |
| pGKK360 | ΔnhaB in pKAS154 | This work |
| pGKK361 | Δclc in pKAS154 | This work |
| pGKK362 | ΔnhaB-lacZ in pKAS154 | This work |
| pGKK364 | Δclc-lacZ in pKAS154 | This work |
| pGKK368 | Δfnr in pKAS154 | This work |
| pKAS359 | cadC-lacZ in pRS415 | This work |
| pWEL92 | ΔVC0770 in pKAS154 | This work |
| pWEL93 | ΔVC0770-lacZ in pKAS154 | This work |
| pWEL134 | ΔcadC in pKAS154 | This work |
| pWEL135 | ΔcadC-lacZ in pKAS154 | This work |
| pWEL136 | Δcah in pKAS154 | This work |
| pWEL138 | Δcah-lacZ in pKAS154 | This work |
| pWEL151 | ΔarcA in pKAS154 | This work |
| pWEL153 | cadC-lacZ in pKAS180 | This work |