TABLE 4.
Activation of AphB regulated promoter-lacZ fusions by low pH
| Strain | β-Galactosidase activitya |
Fold change | |
|---|---|---|---|
| pH 7.0b | pH 5.5c | ||
| GK1222 (Δclc-lacZ) | 32 ± 1 | 217 ± 7 | 6.8 |
| GK1224 (ΔaphB) | 22 ± 1 | 49 ± 1 | 2.2 |
| WL558 (Δcah-lacZ) | 71 ± 7 | 315 ± 3 | 4.4 |
| WL562 (ΔaphB) | 56 ± 11 | 78 ± 1 | 1.3 |
| KSK725 (ΔtcpPH-lacZ) | 377 ± 56 | 1,631 ± 47 | 4.3 |
| GK138 (ΔaphB) | 122 ± 1 | 226 ± 5 | 1.8 |
| GK1232 (ΔnhaB-lacZ) | 238 ± 5 | 687 ± 10 | 2.9 |
| GK1234 (ΔaphB) | 225 ± 42 | 242 ± 2 | 1.0 |
| KSK2896 (ΔlacZ::cadC-lacZ) | 48 ± 1 | 129 ± 1 | 2.7 |
| KSK2900 (ΔaphB) | 42 ± 1 | 40 ± 1 | 0.95 |
a
Units per OD600 of culture.
b
LB medium buffered with 100 mM Trizma.
c
LB medium buffered with 100 mM MES.