TABLE 5.
Activation of a VC0770 promoter-lacZ fusion by low pH
Strain | β-Galactosidase activitya |
Fold change | |
---|---|---|---|
LB medium, pH 7.0b | LB medium, pH 5.5c | ||
WL344 (ΔVC0770-lacZ) | 2,591 ± 65 | 4,403 ± 42 | 1.7 |
WL346 (ΔaphB) | 79 ± 1 | 51 ± 1 | 0.64 |
M9 medium, pH 7.0 | M9 medium, pH 6.0 | ||
---|---|---|---|
WL344 (ΔVC0770-lacZ) | 210 ± 12 | 1,149 ± 32 | 5.5 |
WL346 (ΔaphB) | 45 ± 2 | 41 ± 5 | 0.91 |
Units per OD600 of culture.
Buffered with 100 mM Trizma.
Buffered with 100 mM MES.