TABLE 6.
Activation of AphB-regulated promoter-lacZ fusions by anaerobiosis
| Strain | β-Galactosidase activitya |
Fold change | |
|---|---|---|---|
| Aerobicb | Anaerobicb | ||
| KSK2896 (ΔlacZ::cadC-lacZ) | 48 ± 1 | 575 ± 23 | 12.0 |
| KSK2900 (ΔaphB) | 42 ± 1 | 68 ± 5 | 1.6 |
| WL558 (Δcah-lacZ) | 71 ± 7 | 849 ± 50 | 11.9 |
| WL562 (ΔaphB) | 56 ± 11 | 86 ± 8 | 1.5 |
| KSK725 (ΔtcpPH-lacZ) | 377 ± 56 | 2,731 ± 25 | 7.2 |
| GK138 (ΔaphB) | 122 ± 1 | 230 ± 23 | 1.9 |
| GK1222 (Δclc-lacZ) | 32 ± 1 | 126 ± 4 | 3.9 |
| GK1224 (ΔaphB) | 22 ± 1 | 35 ± 5 | 1.6 |
| GK1232 (ΔnhaB-lacZ) | 238 ± 5 | 555 ± 69 | 2.3 |
| GK1234 (ΔaphB) | 225 ± 42 | 177 ± 5 | 0.79 |
| WL344 (ΔVC0770-lacZ) | 2,591 ± 65 | 5,665 ± 142 | 2.2 |
| WL346 (ΔaphB) | 79 ± 1 | 89 ± 12 | 1.1 |
a
Units per OD600 of culture.
b
LB medium (pH 7.0) buffered with 100 mM Trizma.