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Expression of Des-GFP under aerobic and anaerobic conditions at 37°C and after the temperature downshift. Strain CM31, bearing the Des-GFP fusion, was cultivated aerobically and anaerobically at 37°C and then shifted to 25°C. The samples for immunoblot analysis were taken at 1-h intervals after temperature downshift, as indicated, and analyzed as described in Materials and Methods.
