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. 2010 Jun 18;192(16):4215–4222. doi: 10.1128/JB.00488-10

FIG. 3.

FIG. 3.

Kinetics of Clostridium difficile spore germination in the presence of taurocholate and glycine. Germination rates were calculated from the linear segment of optical density changes over time. (A) Lineweaver-Burk plots of C. difficile spore germination at various concentrations of taurocholate (8, 10, 14, 20, and 30 mM) and 8 (•), 9 (○), 10 (▪), 13 (□), and 16 (⧫) mM glycine concentrations. (B) Squared Lineweaver-Burk plots of C. difficile spore germination at various concentrations of taurocholate (8, 10, 14, 20, and 30 mM) and 8 (•), 9 (○), 10 (▪), 13 (□), and 16 (⧫) mM glycine concentrations. (C) Hill plot for taurocholate binding at the saturating glycine concentration. (D) Lineweaver-Burk plots of C. difficile spore germination at various concentrations of glycine (8, 10, 12, 14, and 18 mM) and 6 (•), 7 (○), 9 (▪), 12 (□), and 15 (⧫) mM taurocholate concentrations. (E) Squared Lineweaver-Burk plots of C. difficile spore germination at various concentrations of glycine (8, 10, 12, 14, and 18 mM) and 6 (•), 7 (○), 9 (▪), 12 (□), and 15 (⧫) mM taurocholate concentrations. (F) Hill plot for glycine binding at the saturating taurocholate concentration.