TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Important feature(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F′ φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 | Invitrogen |
| BW29427 | hsdR17(rK− mK+)phoA supE44 thi-1 | K. Datsenko and B. L. Wanner, unpublished |
| H1717 | fhuF::lplacMu53 | 27 |
| H1780 | fiu::lplacMu53 fur | 27 |
| M. gryphiswaldense | ||
| R3/S1 | Wild type, but Rifr Smr | 60 |
| MSR-1B | Spontaneous nonmagnetic mutant of MSR-1 | 55 |
| RU-1 | R3/S1 Δfur | This study |
| M. magneticum | ||
| AMB-1 | Wild type | 34 |
| Plasmids | ||
| pGEM-T Easy | Cloning vector; Ampr | Promega |
| pGEMPmamDC | pGEM-T Easy plus PmamDC | This study |
| pGEMPmamAB | pGEM-T Easy plus PmamAB | This study |
| pGEMPmms16 | pGEM-T Easy plus Pmms16 | This study |
| pGEMPrplK | pGEM-T Easy plus PrplK | This study |
| pGEMPfhuF | pGEM-T Easy plus PfhuF | This study |
| pGEMPmgr4079 | pGEM-T Easy plus Pmgr4079 | This study |
| pGEMfurup | pGEM-T Easy plus fur 2-kb upstream region | This study |
| pGEMfurdown | pGEM-T Easy plus fur 2-kb downstream region | This study |
| pJET1.2/blunt | Cloning vector; Ampr | Fermentas |
| pJETEcfur | pJET1.2/blunt plus fur from E. coli | This study |
| pJETamb1009 | pJET1.2/blunt plus amb1009 from M. magneticum | This study |
| pJETamb4460 | pJET1.2/blunt plus amb4460 from M. magneticum | This study |
| pJETmgr1314 | pJET1.2/blunt plus mgr1314 from M. gryphiswaldense | This study |
| pBBR1MCS-2 | Mobilizable broad-host-range vector; Kmr | 36 |
| pBBR1MCS-2fur | pBBR1MCS-2 containing fur | This study |
| pBBR1MCS-5 | Mobilizable broad-host-range vector; Gmr | 36 |
| pBBR1Ptet | Mobilizable broad-host-range vector; Kmr, Ptet | C. Lang, unpublished |
| pBBR1MCS-5Ptet | Mobilizable broad-host-range vector; Gmr, Ptet | This study |
| pBBR1MCS-5Ptet/Ecfur | pBBR1MCS-5Ptet with fur from pJETEcfur | This study |
| pBBR1MCS-5Ptet/amb1009 | pBBR1MCS-5Ptet with fur from pJETamb1009 | This study |
| pBBR1MCS-5Ptet/amb4460 | pBBR1MCS-5Ptet with fur from pJETamb4460 | This study |
| pBBR1MCS-5Ptet/mgr1314 | pBBR1MCS-5Ptet with fur from pJETmgr1314 | This study |
| pCM184 | Broad-host-range allelic exchange vector | 39 |
| pCM184furup | pCM184 with 2-kb fragment from pGEMfurup | This study |
| pCM184Δfur | pCM184furup with 2-kb fragment from pGEMfurdown | This study |