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. 2010 Jun 21;30(16):4060–4076. doi: 10.1128/MCB.01399-09

FIG. 3.

FIG. 3.

Pho4- and energy-dependent remodeling of prepositioned nucleosomes at the PHO5 promoter in vitro. (A) DNase I indirect end-labeling analysis of the PHO5 promoter region in chromatin treated as indicated after preassembly by salt gradient dialysis and incubation with yeast extract (from strain YS27 cpf1) in the presence of energy. The schematic, black dots, vertical bars, stippled lines, ramps, and markers are as in Fig. 2. (B) Same as panel A but with treatment of salt gradient dialysis chromatin as indicated above the lanes. Only for the sample in lane 12 were Pho4 and Pho2 added at the same time as the yeast extract. For all of the samples in lanes 3 to 6, 8 to 11, and 13, salt gradient dialysis chromatin was first treated with yeast extract and energy to yield the pattern shown in lane 3 and then further treated for 1 h at 30°C as indicated. In all DNase I mapping experiments, a range of DNase I concentrations was used, but due to space limitations, only one representative concentration is shown for each condition. acCoA, acetyl-CoA.