FIG. 6.

Little or no Pho4-induced chromatin remodeling at the PHO84 promoter in vitro. DNase I indirect end-labeling analysis of the PHO84 promoter region in chromatin preassembled by salt gradient dialysis, incubated with yeast extract (from strain YS27 cpf1) in the presence of energy and then left untreated or treated with Pho4, as indicated. The schematic on the left corresponds to positioned nucleosomes, as in Fig. 1C. The brace between lanes 4 and 5 shows the region that becomes hypersensitive upon induction in vivo (67). Marker fragments were generated by double digests of ClaI, AgeI, ApaI, and BsrBI, each with SspI. Ramps above the lanes denote increasing DNase I concentrations.