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. 2010 Jun 14;30(16):4035–4044. doi: 10.1128/MCB.00175-10

FIG. 1.

FIG. 1.

PGC-1α inhibits endothelial migration. (A) Quantitative analysis of scratch assays in serum-starved confluent BAEC. Cells were preincubated with EUK-189 (100 μM) for 2 h as indicated and then (right after being scratched) incubated with DETA-NO (62 μM) and 8-Br-cGMP (100 μM) as indicated. Repopulation of the denuded area was monitored by confocal microscopy at the indicated times. (B) MitoSOX Red labeling of fixed MLEC, showing mitochondrial superoxide levels in PGC-1α+/+/PGC-1α−/− cells treated with 100 μM 8-Br-cGMP for 6 h. V, vehicle. (C) Scratch wound healing assays with cells infected with PGC-1α or control adenovirus (MOI of 25) at 24 h before the beginning of the assay. Treatments with DETA-NO and 8-Br-cGMP, as shown in panel A, are shown for comparison. Cell migration was monitored at 18 h. (D and E) A total of 10,000 PGC-1α+/+ or PGC-1α−/− MLEC were seeded in the upper chamber of a Transwell insert and allowed to migrate for 18 h before fixing and labeling for cell nuclei. (D) Quantitative analysis of cross sections (z-axis) of Transwell inserts seeded with PGC-1α+/+ or PGC-1α−/− cells, illustrating the distance migrated. Cells were preincubated with EUK-189 (100 μM) for 2 h as indicated. (E) The chart shows the effect of LY294002 (LY; 10 μM) on the Transwell migration of PGC-1α+/+ and PGC-1α−/− MLEC. #, P < 0.05 versus paired control.