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. 2010 Jun 14;30(16):3956–3969. doi: 10.1128/MCB.00242-10

FIG. 7.

FIG. 7.

Localization and activation of Rap1b at the NE. (A) Localization of GFP-tagged Rap1b, Rap2b, and H-Ras expressed in HEK293 cells, as indicated. All photos presented were from confocal live imaging. Bars, 10 μm. (B) Distribution of GFP-RBDRalGDS in HEK293 cells cotransfected with pcDNA3, Flag-Rap1b, or Flag-Rap2b, as indicated. (C) GTP dependency of the enrichment of GFP-RBDRalGDS on the NE in HEK293 cells cotransfected with GFP-RBDRalGDS and either Rap1b-N17 or Rap1b-V12, as indicated. (D) Inactivation of Rap1b on the NE by RapGAP. GFP-RBDRalGDS and mCherry-Rap1b were cotransfected into HEK293 cells with pcDNA3 (i to iii) or RapGAP (iv to vi), as indicated. (i and iv) mCherry; (ii and v) GFP; (iii and vi) merged images. All the images are representative of cells examined in two to three independent experiments (number of cells examined in each condition > 100).