TABLE 1.

Organisms and plasmids used in this study

Strains or plasmids	Propertiesa	Reference
Strains
Pseudomonas fluorescens
CHA0	Biocontrol bacterium, wild-type strain	43
CHA19	gacS isogenic mutant lacking extracellular toxins	48
Escherichia coli DH5α	Laboratory strain	37
Acanthamoeba castellanii	Soil-dwelling bacterivorous amoeba	5
Plasmids
pME7100	phlA-gfp transcriptional fusion; reporter for 2,4-diacetylphloroglucinol biosynthetic gene expression; Tcr	2
pME7109	pltA-gfp transcriptional fusion; reporter for pyoluteorin biosynthetic gene expression; Kmr	2
pME7116	prnA-gfp transcriptional fusion; reporter for pyrrolnitrin biosynthetic gene expression; Tcr	2
pME7156	hcnA-gfp transcriptional fusion; reporter for hydrogen cyanide biosynthetic gene expression; Tcr	32
pME9012	phlA-mcherry transcriptional fusion; reporter for 2,4-diacetylphloroglucinol biosynthetic gene expression; Kmr	32
pSM1973	Delivery plasmid for the mini Tn7 PrrnBP1 gfp-a cassette; reporter for rrnB ribosomal gene expression; Gmr	23
pUX-BF13	Helper plasmid encoding the Tn7 transposition functions; Apr	3

^aAp^r, ampicillin resistant; Gm^r, gentamicin resistant; Km^r, kanamycin resistant; and Tc^r, tetracycline resistant.