TABLE 1.
Strains or plasmids | Propertiesa | Reference |
---|---|---|
Strains | ||
Pseudomonas fluorescens | ||
CHA0 | Biocontrol bacterium, wild-type strain | 43 |
CHA19 | gacS isogenic mutant lacking extracellular toxins | 48 |
Escherichia coli DH5α | Laboratory strain | 37 |
Acanthamoeba castellanii | Soil-dwelling bacterivorous amoeba | 5 |
Plasmids | ||
pME7100 | phlA-gfp transcriptional fusion; reporter for 2,4-diacetylphloroglucinol biosynthetic gene expression; Tcr | 2 |
pME7109 | pltA-gfp transcriptional fusion; reporter for pyoluteorin biosynthetic gene expression; Kmr | 2 |
pME7116 | prnA-gfp transcriptional fusion; reporter for pyrrolnitrin biosynthetic gene expression; Tcr | 2 |
pME7156 | hcnA-gfp transcriptional fusion; reporter for hydrogen cyanide biosynthetic gene expression; Tcr | 32 |
pME9012 | phlA-mcherry transcriptional fusion; reporter for 2,4-diacetylphloroglucinol biosynthetic gene expression; Kmr | 32 |
pSM1973 | Delivery plasmid for the mini Tn7 PrrnBP1 gfp-a cassette; reporter for rrnB ribosomal gene expression; Gmr | 23 |
pUX-BF13 | Helper plasmid encoding the Tn7 transposition functions; Apr | 3 |
Apr, ampicillin resistant; Gmr, gentamicin resistant; Kmr, kanamycin resistant; and Tcr, tetracycline resistant.