TABLE 3.
Bacterial strains used in this study
| Strain | Description (strain abbreviation used throughout the text) | Reference or source |
|---|---|---|
| 10403S | L. monocytogenes parent strain | 4 |
| FSL S4-039 | L. seeligeri wild-type strain | 51 |
| FSL B2-046 | 10403S ΔprfAa | 34 |
| FSL R3-003 | 10403S Δhly | 24 |
| FSL R3-004 | 10403S ΔplcA | 5 |
| FSL L5-029 | 10403S ΔplcA tRNAArg::pPL2 plcALS52 (ΔplcA plcALS52 strain) | This work |
| FSL L5-030 | 10403S Δhly tRNAArg::pPL2 hlyLS314 (Δhly hlyLS314 strain) | This work |
| FSL L5-032 | 10403S ΔprfA tRNAArg::pPL2 prfALS157 (ΔprfA prfALS157 strain) | This work |
| FSL L5-162 | 10403S ΔprfA tRNAArg::pPL2 prfALS367 (ΔprfA prfALS367 strain) | This work |
| FSL L5-113 | 10403S ΔprfA tRNAArg::pPL2 prfALM356 (ΔprfA prfALM356 strain) | This work |
| FSL L5-160 | 10403S ΔprfA tRNAArg::pPL2 PplcA prfALM356 (ΔprfA PplcA prfALM356 strain) | This workb |
| FSL L5-112 | 10403S ΔprfA tRNAARG::pPL2 P1P2LM356prfALS (ΔprfA P1P2LM356prfALS strain) | This work |
a
This strain carries a 339-bp in-frame deletion in prfA.
b
The plasmid with the PplcA prfALM356 construct that was used to construct the pPL2 plasmid employed to generate this strain was first reported by Wong and Freitag (65).