Skip to main content
. 2010 Jun 11;76(15):5046–5057. doi: 10.1128/AEM.00660-10

FIG. 5.

FIG. 5.

Direct disruption of the uvrBA operon by induced competence. (A) Schematic representation of the uvrBA operon from wild-type B. licheniformis (WT) and the uvrBA mutant (MT). SwrB, motility/swarming protein; YwkF, hypothetical protein; CsbA, conserved uncharacterized protein; UvrB, excinuclease ABC subunit B; UvrA, excinuclease ABC subunit A; BL05351, pseudogene; YvkN and YvlA, hypothetical proteins; YvlB, uncharacterized conserved protein; ErmC, erythromycin resistance. (B) Transformation efficiencies via induced competence with suicide vector pUCuvrBAEmR. Black bars, total amounts of obtained erythromycin-resistant transformants; gray bars, fraction of the uvrBA mutants. (C) UV sensitivity of the uvrBA mutant strains (1, 2, 3, and 4) compared to that of the wild type.