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. 2010 Jun 4;76(15):4933–4942. doi: 10.1128/AEM.00477-10

FIG. 4.

FIG. 4.

HPLC and ESI-MS spectrometry analysis of the AidH-catalyzed HHL product. (A) HPLC elution profiles of the reaction buffer (top), reaction buffer containing N-hexanoyl-l-homoserine lactone (HHL) (middle), or AidH-digested HHL products in the reaction buffer (bottom). The HHL peak eluted at 25.4 min (middle); the product peak eluted at 22.1 min (bottom). mAU, milli-absorbance unit. (B) HPLC elution profiles of lactonolysis solution (top), reaction solution containing undigested HHL (middle), and the lactonolysis reaction mixture (bottom). Both the lactonolysis product and AidH enzymatically digested HHL appeared with a retention time of 22.1 min. (C) ESI-MS analysis of the hydrolysis product of HHL. The fraction at 22.1 min from HPLC was collected and analyzed by ESI-MS as described in Materials and Methods.