TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| Ochrobactrum sp. | ||
| T63 | Apr; wild type | This study |
| T63ΔAidH | Apr; aidH deletion mutant | This study |
| T63-AidH | Apr; T63ΔAidH harboring pB8C-1 | This study |
| Agrobacterium tumefaciens NTL4(pZLR4) | Gmr; A. tumefaciens NT1 derivative harboring a traG::lacZ reporter fusion | 7 |
| Escherichia coli | ||
| DH5α | φ80 lacZΔM15 Δ(lacZYA-argF)U169 hsdR17 recA1 endA1 thi-1 | 18 |
| BL21(DE3) | Expression strain | Novagen |
| Pectobacterium carotovorum subsp. carotovorum | ||
| Z3-3 | Wild type | Laboratory stock |
| Z3-3(pBBR1MCS-2) | Kmr; strain Z3-3 harboring pBBR1MCS-2 | This study |
| Z3-3(pB8C-1) | Kmr; strain Z3-3 harboring pB8C-1 | This study |
| Pseudomonas fluorescens | ||
| 2P24 | Apr; wild type | 56 |
| 2P24(pRK415) | Apr Tcr; strain 2P24 harboring pRK415 | This study |
| 2P24(pR8C-1) | Apr Tcr; strain 2P24 harboring pR8C-1 | This study |
| Plasmids | ||
| pBluescript II SK(+) | Apr; ColE1 origin | Stratagene |
| p8C-1 | Apr; pBluescript harboring a 2.3-kb HindIII fragment with the aidH gene | This study |
| pBBR1MCS-2 | Kmr; Escherichia-Pseudomonas shuttle vector | 27 |
| pB8C-1 | Kmr; pBBR1MCS-2 harboring a 2.3-kb HindIII fragment with the aidH gene | This study |
| pSR47S | Kmr; mobRP4 oriR6K sacB | 1 |
| p47SΔAidH | Kmr; suicide plasmid pSR47S carrying a deleted aidH gene | This study |
| pLAFR5 | Tcr; oriV cosmid | 25 |
| pET-22b(+) | Apr; expression vector | Novagen |
| pET-AidH | Apr; pET-22b(+) carrying the aidH gene | This study |
| pHSG399 | Cmr; ColE1 origin | TaKaRa |
| p399-AidH | Cmr; pHSG399 carrying the aidH gene | This study |
| pRK415 | Tcr; IncP1 replicon, polylinker of pUC19; Mob+ | 25 |
| pR8C-1 | Tcr; pRK415 harboring a 2.3-kb HindIII fragment with the aidH gene | This study |
a
Apr, Cmr, Gmr, Kmr, and Tcr indicate resistance to ampicillin, chloramphenicol, gentamicin, kanamycin, and tetracycline, respectively.