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Real-time PCR primers for mouse GAPDH and the T. gondii B1 genes
| Genea | Primer/probe name | Sequence (5′→3′) | Nucleotide position | Amplicon size (bp) |
|---|---|---|---|---|
| Mouse GAPDH | GAPDH-ENV for | TCA TCT CCG CCC CTT CTG | 2575-2592 | 54 |
| GAPDH-ENV rev | TCG TGG TTC ACA CCC ATC AC | 2610-2629 | ||
| VIC-GAPDH-MGB | CGA TGC CCC CAT GTT | 2594-2608 | ||
| T. gondii B1b | Tg-TX2F for | CTA GTA TCG TGC GGC AAT GTG | 531-551 | 62 |
| Tg-TX2R rev | GGC AGC GTC TCT TCC TCT TTT | 572-592 | ||
| FAM-TGTX2M1-MGB | CCA CCT CGC CTC TTG G | 552-567 |
