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. 2010 Jun 11;76(15):5140–5147. doi: 10.1128/AEM.00153-10

TABLE 2.

Primer and probe sequences used for RT-qPCRa

Gene Primer/probe Sequence (5′→3′) Amplicon size (bp)
ACT1 TgACT1 for ACA TCA AGG AGA AGC TTT GCT ACA 60
TgACT1 rev TCA GCC GCC TTC ATT TCC
VIC-ACT1-MGB CGC CCT CGA CTT C
SporoSAG SporoSAG for GCG GAG ACA AGC GTT TTT TT 124
SporoSAG rev AGC CTG TGG CTG CGC TTT
FAM-SporoSAG-MGB CCT ATG CCA AAG AAC
a

Reference 60.