TABLE 5.
Summary of tissue resultsc
| Tissue type | qPCR parametera | Unexposed mice | Asymptomatic mice | Moribund miceb |
|---|---|---|---|---|
| Brain | B1 mean CT ± SD | 39.7 ± 0.5 | 39.9 ± 0.3 | 28.8 ± 2.2 |
| GAPDH mean CT ± SD | 22.1 ± 0.9 | 22.1 ± 1.5 | 21.9 ± 1.2 | |
| n | 15 | 263 | 88 | |
| Spleen | B1 mean CT ± SD | NA | 40.1 ± 0.1 | 30.9 ± 3.1 |
| GAPDH mean CT ± SD | NA | 19.9 ± 1.3 | 19.0 ± 2.1 | |
| n | NA | 6 | 3 | |
| Lung | B1 mean CT | NA | NA | 28.4 |
| GAPDH mean CT | NA | NA | 19.4 | |
| n | NA | NA | 1 |
a
Tissue results are averages from triplicate duplex qPCRs. Undetermined results were given a value of 40.1. Moribund mice were positive for T. gondii if they had a mean B1 CT value of ≤34. Asymptomatic mice were negative if they had a mean B1 CT value of ≥38.7. Primary determination was by brain tissue and, if required, by spleen and then by lung.
b
Seven moribund mice did not have T. gondii and were not included in the analysis (data not shown).
c
NA, no tissues analyzed.