TABLE 1.
Isolation sources and conditions, seawater dependency, and molecular taxonomy based on 16S rRNA gene similarity of the 27 actinomycete isolates used in this studya
| Isolate no. | Source | Sample no. | Depth (in.) | Isolation medium | Sample pretreatment | Obligatory seawater dependence | Organism with greatest 16S rRNA gene similarity (% similarity) [GenBank accession no.] |
|---|---|---|---|---|---|---|---|
| TFS59-23 | Sediment | 4 | 24.6 | 13 | None | + | Streptomyces sp. HV10 (98) [FJ222814] |
| TFS60-19 | Sediment | 9 | 102 | 15 | None | + | Saccharomonospora caesia (98) [Z38022] |
| TFS61-05 | Sediment | 4 | 24.6 | 15 | None | + | Rhodococcus marinonascens (99) [NR_026183] |
| TFS65-03 | Sediment | 4 | 24.6 | 13 | None | + | Nocardiopsis sp. TFS 806 (99) [EF216368] |
| TFS65-07 | Sediment | 4 | 24.6 | 15 | None | − | Prauseria sp. TUT1202 (99) [AB188209] |
| TFS65-13 | Sediment | 4 | 24.6 | 15 | None | − | Streptomyces sp. CNS-579_SD06 (97) [EU214954] |
| TFS65-24 | Sediment | 4 | 24.6 | 16 | None | + | Nocardiopsis sp. TFS 91 (99) [EF216363] |
| TFS66-01 | Sediment | 4 | 24.6 | 15 | None | + | Streptomycetaceae bacterium CNQ719 (98) [AY464544] |
| TFS72-17 | Sediment | 9 | 102 | 17 | Rehydration, centrifugation | − | Nocardiopsis sp. TFS 806 (99) [EF216368.1] |
| TFS73-15 | Sediment | 4 | 24.6 | 17 | Rehydration, centrifugation | + | Nocardiopsis sp. 13634A (99) [EU741113] |
| TFS74-05 | Sediment | 11 | 326 | 20 | Rehydration, centrifugation | + | Nocardiopsis sp. TFS 806 (99) [EF216368] |
| TFS76-05 | Sediment | 9 | 102 | 15 | Filter method | + | Micromonospora auratinigra (98) [AB159779] |
| TFS77-24 | Sediment | 9 | 102 | 16 | Filter method | + | Nocardiopsis sp. TFS 91 (99) [EF216363] |
| TFS79-01 | Sediment | 9 | 102 | 15 | Filter method | − | Streptomyces sp. CNQ-027_SD01 (98) [EU214912] |
| TFS79-06 | Sediment | 2A | 10 | 15 | Filter method | − | Saccharomonospora caesia (99) [Z38022] |
| TFS79-15 | Sediment | 2A | 10 | 17 | Rehydration, centrifugation | − | Actinomycetales bacterium HPA72 (99) [DQ144231] |
| TFS83-14 | Sediment | 2A | 10 | 15 | Filter method | + | Micromonospora auratinigra (98) [AB159779] |
| TFS84-03 | Sediment | 4 | 24.6 | 7b | None | + | Micromonospora sp. HBUM80369 (99) [EU119228] |
| TSI115-07 | Sponge | 3 | 57.2 | 17 | None | + | Micromonospora chokoriensis (99) [AB241454] |
| TSI116-04 | Sponge | 3 | 57.2 | 4 | None | + | Streptomyces sp. CNQ-027_SD01 (98) [EU214912] |
| TSI116-13 | Sponge | 4 | 121.4 | 4 | None | + | Pseudonocardia petroleophila IMSNU 22072T (99) [AJ252828] |
| TSI119-11 | Sponge | 4 | 121.6 | 19 | None | + | Micromonospora chokoriensis 173803 (100) [EU570342] |
| TSI124-17 | Sponge | 6 | 62.9 | 15 | Filter method | + | Streptosporangium sp. TFS 224 (99) [EF212016] |
| TSI125-23 | Sponge | 4 | 121.4 | 17 | None | + | Micromonospora matsumotoense IMSNU 22003 (99) [NR_025015] |
| TSI127-17 | Sponge | 5 | 60 | 18 | None | + | Actinoalloteichus hymeniacidonis HPA177 (98) [DQ144222] |
| TSI129-12 | Sponge | 3 | 57.2 | 18 | None | + | Streptosporangium amethystogenes DSM43179T (99) [X89935] |
| TSI129-23 | Sponge | 6 | 62.9 | 15 | None | + | Streptosporangium amethystogenes DSM43179T (99) [X89935] |
a
For detailed information about sampling locations and origins (Tables S1 and S2), isolation procedures (Table S3), and isolation media, see the supplemental material.