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Effects of temperature (A) and pH (B) on Pel activity. Enzyme activities were measured using a standard reaction mixture containing 1 g/liter PGA as the substrate. The effect of pH was checked at a constant temperature of 37°C. The effect of temperature was studied at the constant optimum pH of each Pel (8 for Pel-66 and Pel-90 and 7.5 for Pel-22). Each result reported is the average of three independent experiments, and the error bars indicate the standard deviations.
