FIG. 3.

Analysis of the different end products of Pel activities. Enzymes were added to the standard reaction mixture at a concentration of 5 U/ml, and the preparations were incubated at 45°C for 10 h. For a combination of two Pels, 2.5 U/ml of each enzyme was used. Twenty microliters of reaction products was run on the chromatogram sheet (Merck, France). Lanes A and B are the standards. Lane A, galacturonic acid; lane B, di- and trigalacturonic acids. The negative control was prepared under the same conditions as the assays, except that no enzyme was added to the reaction mixture. The arrows indicate the end products of the synergistic action. Blacks arrows, individual action; white arrows, combined action.