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Km and Vmax values for each Pel with polygalacturonate and pectin (45% esterification) as substratesa
| Pel | PGA |
Pectin |
||
|---|---|---|---|---|
| Km | Vmax | Km | Vmax | |
| Pel-22 | 0.045 | 1.41 | 0.043 | 1.45 |
| Pel-66 | 0.1 | 667 | 0.1 | 714 |
| Pel-90 | 0.125 | 909.9 | 0.111 | 1136.36 |
The initial velocity for the reactions was determined at different polygalacturonate and pectin concentrations under standard conditions. Lineweaver-Burk transformation of the data gave Km (g/liter) and Vmax (μmol/min/mg) values for each enzyme.
