TABLE 1.

Primers used in this study

Primer	Gene targeta	Sequence (5′ to 3′)b	Tmc (°C)	Reference
DSR1F	dsrA	ACS CAC TGG AAG CAC G	57.0	Wagner et al. (17)
DSR4R	dsrB	GTG TAG CAG TTA CCG CA	51.1	Wagner et al. (17)
DSR1334R	dsrA	TYT TCC ATC CAC CAR TCC	57.2	This study
DSR1Fmixd
DSR1F	dsrA	ACS CAC TGG AAG CAC G		Wagner et al. (17)
DSR1Fa	dsrA	ACC CAY TGG AAA CAC G	53.0	Loy et al. (6a)
DSR1Fb	dsrA	GGC CAC TGG AAG CAC G	59.9	Loy et al. (6a)
DSR1Fc	dsrA	ACC CAT TGG AAA CAT G	49.8	Zverlov et al. (20)
DSR1Fd	dsrA	ACT CAC TGG AAG CAC G	50.4	Zverlov et al. (20)
DSR4Rmixe
DSR4R	dsrB	GTG TAG CAG TTA CCG CA		Wagner et al. (17)
DSR4Ra	dsrB	GTG TAA CAG TTT CCA CA	43.9	Loy et al. (6a)
DSR4Rb	dsrB	GTG TAA CAG TTA CCG CA	47.8	Loy et al. (6a)
DSR4Rc	dsrB	GTG TAG CAG TTK CCG CA	56.0	Loy et al. (6a)
DSR4Rd	dsrB	GTG TAG CAG TTA CCA CA	44.3	Zverlov et al. (20)
DSR4Re	dsrB	GTG TAA CAG TTA CCA CA	40.6	Zverlov et al. (20)

^aPrimer annealing position according to Desulfovibrio vulgaris Hildenborough strain (GenBank accession no. U16723).

^bWobble positions are shown as follows: S = G or C, Y = C or T, R = A or G, and K = G or T.

^cT_m, melting temperature.

^dThe final primer mixture is an equimolar mixture of each variant (50 μM) of the following primers: DSR1F, DSR1Fa, DSR1Fb, DSR1Fc, and DSR1Fd.

^eThe final primer mixture is an equimolar mixture of each variant (50 μM) of the following primers: DSR4R, DSR4Ra, DSR4Rb, DSR4Rc, DSR4Rd, and DSR4Re.