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Invasion of C. sakazakii into Caco-2 and INT-407 cells after pretreatment with EGTA. The monolayers were either 4 to 7 or 14 to 21 days old and were pretreated (W/ EGTA) or not pretreated (W/O EGTA) with 3 mM EGTA. Internalized bacteria were determined as described in the legend to Fig. 2 using Caco-2 (A and B) and INT-407 (C and D) cells. Percent invasion was determined by comparison to the non-EGTA-treated WT. Note that the scales in the y axes are different.
