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In vivo animal study. Isogenic C. sakazakii strains grown aerobically were used to infect 2- or 3-day-old rat pups. A group of five rats were orally inoculated with 5 × 109 CFU of C. sakazakii. To analyze bacterial colonization in different organs, the rat pups were anesthetized 24 h postinfection. The spleens and livers were removed, homogenized, and then plated on LB agar plates.
